Single Cell Genome Amplification Kit

The new amplification methodology primary template directed amplification or pta employs controlled reaction parameters to reproducibly recover greater than 95 of the genome of single cells and limited dna input samples with best in class uniformity and accuracy. Genomeplex single cell whole genome amplification kit utilizes a proprietary technology based on random fragmentation of genomic dna and conversion of the resulting small fragments to pcr amplifiable library molecules flanked by universal priming sites. For whole genome amplification wga from single eukaryotic cells limited samples or purified genomic dna repli g midi kit for highly uniform whole genome amplification from small or precious samples.

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Single cell genome amplification kit.

The single cell procedure differs very little from the previously described genomeplex system but for three procedural changes. 4bb trueprime single cell wga kit uses a novel and reliable method to achieve accurate genome amplification from single cells or fewer cells. Single cell genomiphi dna amplification kit has been optimized to wholly amplify genomic dna from as little as a single cell generating micrograms of high quality dna for use in downstream applications. 4bb trueprime single cell wga kit uses alkaline incubation to allow cell lysis and dna denaturation of genomic dna with very low dna fragmentation.

Wga is achieved by pcr amplification of the library molecules using universal oligonucleotide primers. This results in amplified dna with high integrity and fragment length so. The method is based on the malbac multiple annealing and looping based amplification cycles technology 1 which carries out close to linear pre amplification cycles of the entire genome using a mixture of highly processive dna polymerases with strand displacement activity followed by an exponential amplification by pcr to a sufficient amount for various downstream analyses. Amplification of a few cells instead of one can improve total coverage breadth if your experimental design allows this.

2 the primers that have been optimized for increased sensitivity. Hundreds of cells or sub ng dna. Introduction the single cell wga kit efficiently amplifies genome dna from single cells or equivalent dna amount to produce 2 4 micrograms of amplified dna in about four hours. Whole genome amplification from a single cell is now possible with our optimized genomeplex single cell whole genome amplification kit.

Sample to insight for single cell wga 16 ideally suited for whole genome amplification from eukaryotic or bacterial single cells analyzing aneuploidy and sub chromosomal copy number variations sequence variation analysis snv structural variants in single cells sensitive microbial applications downstream analysis using acgh pcr or ngs multiple analyses from a single cell bio banking the genomic content of a single cell repli g single cell kit single cell genomics by qiagen 2016. The kit can also be used when the input amount of dna is low e g. At very low levels of input dna successful amplification relies on reagent quality. The single cell wga kit generates highly uniform amplification across the entire genome.

1 the kit includes a robust optimized cell lysis protocol that is incorporated into the fragmentation steps.

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